Imaging options
The FRAP imaging options are available on the FRAP Benchtop Rock Imager or Rock Imager 1000 with a FRAP imaging method option. To support your imaging performance, FRAP Configuration provides some optional settings such as Imaging Settings, Exposure Settings, and Bright Field Focusing. To configure these settings, on the File menu, click FRAP Configurations..., and click Imaging.
Imaging options
Imaging SettingsAn imaging setting is a collection of settings that tells Rock Imager how an experiment plate should be imaged. These are defined in Rock Maker. The specific imaging setting that has been set in Rock Maker displays on the Single Drop Full Recovery field.
Exposure SettingsThe exposure settings affects images brightness. It enables you to adjust some settings, such as, fluorescent light, gamma, exposure, and gain.
| Option | Description |
|---|---|
| Fluoro Light | Changing this value affects the fluorescence signal intensity. 100% is the most intense. |
| Gamma | This field affects the contrast of the midtones in the image. When you modify this setting, only the midtones are affected, without affecting black and white. |
| Exposure | The Exposure field populates with the amount of time in milliseconds that the camera's shutter remains open when capturing an image, which determines the amount of light allowed to fall on the camera's sensor affecting the image's brightness. |
| Gain | The gain setting is used to amplify signal strength in low-light conditions, increase the sensitivity and make the image brighter. Increasing the amount of gain can also increase the noise in an image. |
Focusing OptionsIt enables you to specify some settings such as condenser, bright field, gamma, exposure, and gain that will be used for bright field focusing.
| Option | Description |
|---|---|
| Auto focus - Condenser | The condenser collects light from the Kohler light source and concentrates it onto the well being examined. Values range from 0 - 100%. At 0%, the iris is fully open, and a cone of light is concentrated at a wide angle. At 100%, the iris is closed, and the light is concentrated in a column of light as opposed to a cone. Adjusting the condenser value can improve image contrast. Increasing the condenser angle increases the contrast. |
| Bright Field | This value sets the brightness level of the bright-field illumination source that passes through the drop from below. At 0%, the light is turned off, and at 100% the light is at maximum brightness. With bright-field illumination, light is passed through the specimen under the microscope so that the specimen appears dark against a bright background. |
| Gamma | See Gamma in the Exposure Settings area. |
| Exposure | See Exposure in the Exposure Settings area. |
| Gain | See Gain in the Exposure Settings area. |
|
|
|
RIC-V214R114
|
